Tissue Culture Room Guidelines

Ensure there are no open doors or windows.

Tissue culture room guidelines.

Reduce foot traffic into the room as much as possible. Typically the culture room for growth of plant tissue cultures should have a temperature between 15 and 30 c with a. Nfpa 45 chapter 6 3 4 and 6 9 1. The tc room technical officer in charge to in charge mr.

These environmental factors may influence the growth and differentiation process directly. Air shall flow from the hallway to the inner lab negative to the hallway. Culture or indirectly by affecting their response in subsequent generations. Drafts bring in bugs and disrupt your hood s airflow.

All room air shall be exhausted through ducts to the outside of the building and not recirculated within the building. Lau king kwan kenneth is responsible for the management maintenance and coordination among users of the tc. Room air which is exhausted to a common plenum is not acceptable. All types of tissue cultures should be incubated under conditions of well controlled temperature humidity air circulation and light quality and duration.

The tc room technical officer in charge to in charge mr. Once the cells have been cultured they can be used in many different types of studies including studies on the biochemistry of the tissues and the. Requirements for tissue culture rooms. Tissue culture room checklist after entering the tissue culture room.

2018 a principal investigator pi is required to conduct a risk assessment in support of any proposed work that is subject to review and approval by the institutional biosafety committee ibc. Unl biosafety guidelines rev. Ideally lab coats should live in the room and never be removed. Tissue culture is a way of getting more cells from the tissue by growing them off of the organism.

Guidelines for tissue culture room the department of chemistry has five tissue culture tc rooms room p2411 p2423 p2427 g1517 g1703 for cell culture experiments. Lau king kwan kenneth is responsible for. For an at a glance way to find what equipment is right for you download the single sheet biochambers tissue culture comparison chart. This sop provides guidance on conducting a risk assessment when the work involves mammalian cell and tissue cultures.

Put on a lab coat and gloves. Biochambers chambers and rooms are available with a full full range of options to suit your research needs and sizes to give you the largest growth area within the available space in your lab. Nfpa 99 chapter 5 4 3 2. Protoplast cultures low density cell suspension cultures and anther cultures are particularly sensitive to environmental cultural condition.

Nih design policy and guidelines research laboratory 1996 d 7 7.